Welcome to the Chapleau Lab
Dr. Chapleau's research focuses on mechanisms of autonomic regulation in health and disease including effects of aging, hypertension, hypercholesterolemia, cardiomyopathy, myocardial infarction (MI), heart failure, and muscular dystrophy. Key hypotheses involve the roles of acid-sensing ion channels in sensory transduction, reactive oxygen species and angiotensin in sensory and sympathetic signaling, and oxidative stress in autonomic dysregulation. Experimental approaches include assessment of the cellular and molecular basis of sensory transduction and neuronal excitability in baroreceptor and sympathetic neurons using patch-clamp, imaging, and molecular techniques; recording of baroreceptor and sympathetic nerve activity in vivo; and assessment of cardiovascular and autonomic function in conscious mice using radiotelemetry.
Increased receptor activity-modifying protein 1 in the nervous system is sufficient to protect against autonomic dysregulation and hypertension.
Sabharwal R, Mason BN, Kuburas A, Abboud FM, Russo AF, Chapleau MW.
J Cereb Blood Flow Metab. 2018 Jan 1:271678X17751352. doi: 10.1177/0271678X17751352.
The volume-regulated anion channel (LRRC8) in nodose neurons is sensitive to acidic pH.
Wang R, Lu Y, Gunasekar S, Zhang Y, Benson CJ, Chapleau MW, Sah R, Abboud FM.
JCI Insight. 2017 Mar 9;2(5):e90632. doi: 10.1172/jci.insight.90632.
Abnormal CD161+ immune cells and retinoic acid receptor-related orphan receptor γt-mediate enhanced IL-17F expression in the setting of genetic hypertension.
Singh MV, Cicha MZ, Kumar S, Meyerholz DK, Irani K, Chapleau MW, Abboud FM.
J Allergy Clin Immunol. 2017 Sep;140(3):809-821.e3. doi: 10.1016/j.jaci.2016.11.039. Epub 2017 Jan 16.
Nicotine Mediates CD161a+ Renal Macrophage Infiltration and Premature Hypertension in the Spontaneously Hypertensive Rat.
Harwani SC, Ratcliff J, Sutterwala FS, Ballas ZK, Meyerholz DK, Chapleau MW, Abboud FM.
Circ Res. 2016 Oct 28;119(10):1101-1115. Epub 2016 Sep 22.
Chronic vagal nerve stimulation prevents high-salt diet-induced endothelial dysfunction and aortic stiffening in stroke-prone spontaneously hypertensive rats.
Chapleau MW, Rotella DL, Reho JJ, Rahmouni K, Stauss HM.
Am J Physiol Heart Circ Physiol. 2016 Jul 1;311(1):H276-85. doi: 10.1152/ajpheart.00043.2016. Epub 2016 May 20.
Fibrotic Aortic Valve Stenosis in Hypercholesterolemic/Hypertensive Mice.
Chu Y, Lund DD, Doshi H, Keen HL, Knudtson KL, Funk ND, Shao JQ, Cheng J, Hajj GP, Zimmerman KA, Davis MK, Brooks RM, Chapleau MW, Sigmund CD, Weiss RM, Heistad DD.
Arterioscler Thromb Vasc Biol. 2016 Mar;36(3):466-74. doi: 10.1161/ATVBAHA.115.306912. Epub 2016 Jan 14.
Exercise prevents development of autonomic dysregulation and hyperalgesia in a mouse model of chronic muscle pain.
Sabharwal R, Rasmussen L, Sluka KA, Chapleau MW.
Pain. 2016 Feb;157(2):387-98. doi: 10.1097/j.pain.0000000000000330.